性别:男
职称:副教授
学历: 理学博士
电子邮件: [email protected]
导师类型: 硕士生导师
学科方向:
071010-生物化学与分子生物学
0860-生物与医药
研究方向
基因编辑技术开发、基因编辑治疗、分子定向进化
个人经历
2006.09-2010.06 色情小说网站
，获理学学士学位
2010.09-2015.06 色情小说网站
，获理学博士学位
2015.07-2018.06 色情小说网站
特聘副研究员
2018.07-至今 色情小说网站
副教授
讲授课程
生理学实验
学术成就
作为第一或通讯作者（含共同）在Nature Communications, Protein & Cell, Molecular Therapy, Small Methods, Advanced Science, Cell Discovery, Science Bulletin, Journal of Genetics and Genomics等SCI杂志发表论文20余篇，其中，ESI高引论文4篇。研究工作入选Nature杂志2015年“365 days: Nature'10”，并被Nature杂志2017年“2017 in news: The science events that shaped the year”报道引用。文章总引用次数超过2000次（Google Scholar）。
1. 开发了能够装载在AAV病毒中进行体内递送的LDE-CjABE, Split-ABE和Split-PE系统。
2. 开发了能够检测腺嘌呤单碱基编辑器（ABE）在全基因组范围内脱靶位点的新方法（EndoV-seq），以及能够系统检测Cas核酸酶、单碱基编辑器（包括CBE和ABE）和先导编辑器（PE）脱靶位点的新方法(CROss-seq）。
3. 开发了基于Type I-F CRISPR-Cas系统的高效转录激活工具PaeCascade-VPR和基于相分离辅助激活的高效转录激活工具dCas9-VPRF。
承担课题
1. 广东省自然科学基金博士启动项目，2016.01-2018.12，10万元，主持
2. 高校基本科研业务费青年教师培育项目，2016.01-2018.12，15万元，主持
3. 国家自然科学基金青年项目，2017.01-2019.12，20万元，主持
4. 广州市科技计划自由申请项目，2017.05-2020.04，20万元，主持
5. 广东省特支计划本土创新团队, 2020.07-2025.07，180万元，课题组长
6. 国家重点研发计划青年项目，2024.12-2027.12，338.8万元，课题组长
7. 国家自然科学基金面上项目，2025.01-2028.12，50万元，主持
8. 广东省自然科学基金面上项目，2025.01-2027.12，10万元，主持
论文专著
1. Chen YX#, Kang R#, Jiang YL#, Zheng Q#, Yang Y, Liu JQ, Wu GL, Zhao WJ, Li Z, Peng CX, Zhang PF, Peng F, Liu QY, Hu SH, Luo X, Wu GF, Cui KX, Huang JJ, Wang YM, Songyang Z, Liang PP*. (2024). Rationally designed Campylobacter jejuni Cas9 enables efficient gene activation and base editing. Molecular Therapy:Nucleic Acids, doi: 10.1016/j.omtn.2024.102366. 杂志同期发表评论
2. Liu JQ#, Chen YX#, Nong BT, Luo X, Cui KX, Li Z, Zhang PF, Tan WQ, Yang Y, Ma WB, Liang PP*, Songyang Z*. (2023). CRISPR-assisted transcription activation by phase-separation proteins. Protein & Cell, 14, 874-887. 封面文章
3. Li Y#, Zhi SY#, Wu T#, Chen HX, Kang R, Ma DZ, Songyang Z, He C*, Liang PP*, Luo GZ*. (2023). Systematic identification of CRISPR off-target effects by CROss-seq. Protein & Cell, 14, 299-303.
4. Zhang XY#, Li T, Ou JP*, Huang JJ*, Liang PP*. (2022). Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing. Protein & Cell, 13, 316-335.
5. Zhi SY#, Chen YX#, Wu GL, Wen JK, Wu JN, Liu QY, Li Y, Kang R, Hu SH, Wang JH, Liang PP*, Huang JJ*. (2021). Dual-AAV delivering split prime editor system for in vivo genome editing. Molecular Therapy, doi: 10.1016/j.ymthe.2021.07.011. ESI高引论文
6. Wen JK#, Cao TQ#, Wu JN, Chen YX, Zhi SY, Huang YM, Zheng PL, Wu GL, Aagaard L, Zhong JX*, Liang PP*, Huang JJ*. (2021). Single AAV-mediated CRISPR/Nme2Cas9 efficiently reduces mutant hTTR expression in a transgenic mouse model of transthyretin amyloidosis. Molecular Therapy, doi: 10.1016/j.ymthe.2021.05.010.
7. Liu T#, Wang LQ#, Liang PP#, Wang XJ, Liu YK, Cai J, She YC, Wang D, Wang Z, Guo ZY, Bates S, Xia XJ, Huang JJ, Cui J*. (2020). USP19 suppresses inflammation and promotes M2-like macrophage polarization by manipulating NLRP3 function via autophagy. Cellular & Molecular Immunology, doi: 10.1038/s41423-020-00567-7.
8. Chen YX#, Liu JQ#, Zhi SY, Zheng Q, Ma WB, Huang JJ, Liu YZ, Liu D, Liang PP*, Songyang Z*. (2020). Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells. Nature Communications 11, 1.
9. Chen YX#, Zhi SY#, Liu WL# , Wen JK#, Hu SH, Cao TQ, Sun HW, Li Y, Huang L, Liu YZ, Liang PP*, Huang JJ*. (2020). Development of highly efficient dual-AAV split adenosine base editor for in vivo gene therapy. Small Methods, doi: 10.1002/smtd.202000309.
10. Sun HW#, Zhi SY#, Wu GF, Wu GL, Cao TQ, Hao H, Songyang Z*, Liang PP*, Huang JJ*. (2020). Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins. Journal of Genetics and Genomics, doi: 10.1016/j.jgg.2020.05.005.
11. Chen MX#, Zhao ZY#, Meng QC#, Liang PP#, Su ZX, Wu YX, Huang JJ, Cui J*. (2019). TRIM14 promotes noncanonical NF-kB activation by modulating p100/p52 stability via selective autophagy. Advanced Science, doi: 10.1002/advs.201901261.
12. Liang PP#, Wen JK, Huang JJ*. (2019). Off-target effects of cytidine base editors and adenine base editor: What can we do?. Journal of Genetics and Genomics, doi: 10.1016/j.jgg.2019.09.004. 特邀评论
13. Liang PP#, Huang JJ*. (2019). Off-target challenge for base editor-mediated genome editing. Cell Biology and Toxicology, doi: 10.1007/s10565-019-09474-8. 特邀评论
14. Lin ZH#, Liang PP#, Yao ZK, Chen YX, Zhang XY, Huang R, Zhang Z, Li MY, Ma WB, Zheng HY, Cao SB, Shi G, Zhao XY, Songyang Z*, Huang JJ*. (2019). A novel undifferentiated spermatogonia-specific surface protein (USSP1) in neonatal mice. Science Bulletin, doi: 10.1016/j.scib.2019.03.022.
15. Huang Y#, Ding CH#, Liang PP#, Li DD, Tang Y, Meng W, Sun HW, Lu HY, Chen Y, Chen XY, Huang QS, Fang JP, Zhou CQ*, Yang SH*, Huang JJ*. (2019). HBB-deficient Macaca fascicularis monkey presents with human β-thalassemia. Protein & Cell, doi: 10.1007/s13238-019-0627-y.
16. Liang PP#, Xie XW#, Zhi SY, Sun HW, Zhang XY, Chen Y, Chen YX, Xiong YY, Ma WB, Liu D, Huang JJ*, Songyang Z*. (2019). Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nature Communications 10, 67. ESI高引论文
17. Liang PP#*, Sun HW#, Zhang XY#, Xie XW#, Zhang JR, Bai YF, Ouyang XL, Zhi SY, Xiong YY, Ma WB, Liu D, Huang JJ*, Songyang Z*. (2018). Effective and precise adenine base editing in mouse zygotes. Protein & Cell, doi: 10.1007/s13238-018-0566-z. 亮点文章
18. Liang PP#, Ding CH#, Sun HW#, Xie XW#, Xu YW, Zhang XY, Sun Y, Xiong YY, Ma WB, Liu YX, Wang YL, Fang JP, Liu D, Songyang Z*, Zhou CQ*, Huang JJ*. (2017). Correction of beta-thalassemia mutant by base editor in human embryos. Protein & Cell 8, 811-822. ESI高引论文
19. Liang PP#,*, Sun HW#, Sun Y, Zhang XY, Xie XW, Zhang JR, Zhang Z, Chen YX, Ding CH, Xiong YY, Ma WB, Liu D, Huang JJ*, Songyang Z*. (2017). Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein & Cell 8, 601-611. 亮点文章
20. Liang PP#, Zhang XY, Chen YX, Huang JJ*. (2017). Developmental history and application of CRISPR in human disease. Journal of Gene Medicine 19.
21. Chen YX#, Liang PP#, Huang Y#, Li MY, Zhang XY, Ding CH, Feng JY, Zhang Z, Zhang XQ, Gao YZ, Zhang QF, Cao SB, Zheng HY, Liu D, Songyang Z*, Huang JJ*. (2017). Glycerol kinase-like proteins cooperate with Pld6 in regulating sperm mitochondrial sheath formation and male fertility. Cell Discovery 3.
22. Feng JY#, Liang PP#, Chen YX, Zhang XY, Songyang Z, Zheng HY, Cao SB, Huang JJ*. (2017). Testis-specific Lypd9 is dispensable for spermatogenesis in mouse. Molecular Reproduction and Development，doi:10.1002/mrd.22942.
23. Zhang XY#, Liang PP#, Ding CH#, Zhang Z, Zhou JW, Xie XW, Huang R, Sun Y, Sun HW, Zhang JR, Xu YW, Songyang Z*, Huang JJ*. (2016). Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Scientific Reports Uk 6.
24. Liang PP#, Xu YW#, Zhang XY#, Ding CH#, Huang R, Zhang Z, Lv J, Xie XW, Chen YX, Li YJ, Sun Y, Bai YF, Songyang Z, Ma WB, Zhou CQ*, Huang JJ*. (2015). CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein & Cell 6, 363-372. ESI高引论文
